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USP33 Recombinant antibody

USP33 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242535A6

Cat no : 84958-1-RR

Synonyms

Ubiquitin thioesterase 33, Ubiquitin carboxyl-terminal hydrolase 33, KIAA1097, hVDU1, EC:3.4.19.12

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using USP33 Recombinant antibody (84958-1-RR)

    IF Staining of HeLa using 84958-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP33 antibody (84958-1-RR, Clone: 242535A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84958-1-RR targets USP33 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen USP33 fusion protein Ag14268
Full Name ubiquitin specific peptidase 33
Calculated Molecular Weight 942 aa, 107 kDa
GenBank Accession NumberBC016663
Gene Symbol USP33
Gene ID (NCBI) 23032
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP33(Ubiquitin carboxyl-terminal hydrolase 33) is also named as KIAA1097, VDU1 and belongs to the peptidase C19 family. This protein, originally identified as a von Hippel-Lindau tumor suppressor (VHL) protein-interacting deubiquitinating enzyme, binds to the Robo1 receptor, and that USP33 is required for Slit responsiveness in breast cancer cells(PMID:19706539). The localization of USP33 is broadly confined to the secretory pathway, with all variants localizing to endoplasmic reticulum-associated structures(PMID:21801292). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for USP33 antibody 84958-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols