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  • Western Blot (WB) analysis of HUVEC cells using CD93 Recombinant antibody (84945-4-RR)

    WB analysis of HUVEC using 84945-4-RR

    HUVEC cells were subjected to SDS PAGE followed by western blot with 84945-4-RR (CD93 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD93 Recombinant antibody (84945-4-RR)

    IHC staining of human tonsillitis using 84945-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84945-4-RR (CD93 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD93 Recombinant antibody (84945-4-RR)

    IHC staining of human tonsillitis using 84945-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84945-4-RR (CD93 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD93 Recombinant antibody

CD93 {ptg:Brand} Recombinant Antibody for WB, IHC, ELISA
Cat No. 84945-4-RR
Clone No.242464D2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

C1QR1, Matrix-remodeling-associated protein 4, C1qRp, C1qR(p), C1qR

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHUVEC cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84945-4-RR targets CD93 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein Predict reactive species
Full Name CD93 molecule
Calculated Molecular Weight69 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberNM_012072.3
Gene Symbol CD93
Gene ID (NCBI) 22918
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NPY3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD93, also known as C1qR or C1qR(p), is a single-pass type I transmembrane glycoprotein and a member of the group 14 of the C-type lectin-like domain (CTLD) superfamily, which also includes endosialin/CD248, thrombomodulin, and CLEC14A (PMID: 31287944). CD93 consists of a C-type lectin-like domain, five EGF-like repeats, a mucin-like domain, a transmembrane domain and a cytoplasmic domain (PMID: 28912033). CD93 is mainly expressed on endothelial cells and has been reported to be expressed by neurons and various cells of the hematopoietic system, including monocytes, neutrophils, B cells, natural killer cells, platelets and hematopoietic stem cells (PMID: 37443812). CD93 plays a role in various physiological processes including angiogenesis, inflammation, and cell adhesion (PMID: 31287944).

Protocols

Product Specific Protocols
WB protocol for CD93 antibody 84945-4-RRDownload protocol
IHC protocol for CD93 antibody 84945-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols