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  • Western Blot (WB) analysis of various lysates using HGD Recombinant antibody (84931-5-RR)

    WB analysis using 84931-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84931-5-RR (HGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HGD Recombinant antibody (84931-5-RR)

    WB analysis using 84931-5-RR

    rat liver tissue lysates were subjected to SDS PAGE followed by western blot with 84931-5-RR (HGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84931-5-RR

    Affinity and Kinetic Characterization of 84931-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84931-5-RR against Human HGD were performed. The affinity constant is 1.38 nM.

HGD Recombinant antibody

HGD {ptg:Brand} Recombinant Antibody for WB, ELISA
Cat No. 84931-5-RR
Clone No.242485E11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

Homogentisate 1,2-dioxygenase, Homogentisate 1,2 dioxygenase, HGO, EC:1.13.11.5, AKU

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84931-5-RR targets HGD in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HGD fusion protein Ag9544 Predict reactive species
Full Name homogentisate 1,2-dioxygenase (homogentisate oxidase)
Calculated Molecular Weight 37 kDa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC020792
Gene Symbol HGD
Gene ID (NCBI) 3081
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ93099
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Homogentisate1,2-dioxygenase (HGD), also named as HGO, is a mononuclear Fe(II)-dependent oxygenase that catalyzes the third step in the pathway for the catabolism of tyrosine, the conversion of homogentisate (HG) to maleylacetoacetate (MAA) and it can exsit as a dimer or trimer(PMID:14678794). HGD consists of a single type of subunit with no intermolecular disulfide bridges and requires Fe2+ as a cofactor(PMID: 7705358). Defects in HGD are the cause of alkaptonuria (AKU)(PMID:10594001).

Protocols

Product Specific Protocols
WB protocol for HGD antibody 84931-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols