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  • Western Blot (WB) analysis of human placenta tissue using EPB42 Recombinant antibody (84921-3-RR)

    WB analysis of human placenta using 84921-3-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 84921-3-RR (EPB42 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using EPB42 Recombinant antibody (84921-3-RR)

    IHC staining of human placenta using 84921-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84921-3-RR (EPB42 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using EPB42 Recombinant antibody (84921-3-RR)

    IHC staining of human placenta using 84921-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84921-3-RR (EPB42 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEL cells using EPB42 Recombinant antibody (84921-3-RR)

    IF Staining of HEL using 84921-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HEL cells using EPB42 antibody (84921-3-RR, Clone: 242412F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

EPB42 Recombinant antibody

EPB42 {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84921-3-RR
Clone No.242412F4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

242412F4, E42P, Erythrocyte protein 4.2, P4.2, PA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEL cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84921-3-RR targets EPB42 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EPB42 fusion protein Ag23556 Predict reactive species
Full Name erythrocyte membrane protein band 4.2
Observed Molecular Weight77 kDa
GenBank Accession NumberBC096093
Gene Symbol EPB42
Gene ID (NCBI) 2038
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16452
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPB42, also known as Protein 4.2, is amongst the most abundant protein components of the erythrocyte membrane, representing approximately 5% membrane content or ~2.5 × 105 copies/cell. EPB42 binds to the cytoplasmic domain of band 3, which serves as a site for the anchorage of several membrane skeletal proteins(PMID: 19269200, 12049649). EPB42 is a 72 kDa peripheral membrane protein.

Protocols

Product Specific Protocols
WB protocol for EPB42 antibody 84921-3-RRDownload protocol
IHC protocol for EPB42 antibody 84921-3-RRDownload protocol
IF protocol for EPB42 antibody 84921-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols