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CXCR4 Recombinant antibody

CXCR4 Uni-rAbTM Recombinant Antibody for IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

241734B2

Cat no : 84904-1-RR

Synonyms

CXC-R4, CXCR 4, CXC R4, C-X-C chemokine receptor type 4, CD184

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CXCR4 Recombinant antibody (84904-1-RR)

    IF Staining of mouse testis using 84904-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CXCR4 antibody (84904-1-RR, Clone: 241734B2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CXCR4 Recombinant antibody (84904-1-RR)

    IF Staining of Jurkat using 84904-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CXCR4 antibody (84904-1-RR, Clone: 241734B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CXCR4 Recombinant antibody (84904-1-RR)

    IF Staining of Jurkat using 84904-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CXCR4 antibody (84904-1-RR, Clone: 241734B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:125-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84904-1-RR targets CXCR4 in IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name chemokine (C-X-C motif) receptor 4
Calculated Molecular Weight 352 aa, 40 kDa
GenBank Accession NumberBC020968
Gene Symbol CXCR4
Gene ID (NCBI) 7852
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some research, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).

Protocols

Product Specific Protocols
IF protocol for CXCR4 antibody 84904-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols