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  • Western Blot (WB) analysis of various lysates using RPL15 Recombinant antibody (84900-1-RR)

    WB analysis using 84900-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84900-1-RR (RPL15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

RPL15 Recombinant antibody

RPL15 Recombinant Antibody for WB, ELISA
Cat No. 84900-1-RR
Clone No.242029E3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

RPL10, ribosomal protein L15, Large ribosomal subunit protein eL15, EC45, 60S ribosomal protein L15

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, COLO 320 cells, NCI-H1299 cells, mouse colon tissue, rat colon tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84900-1-RR targets RPL15 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPL15 fusion protein Ag9948 Predict reactive species
Full Name ribosomal protein L15
Calculated Molecular Weight 204 aa, 24 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC070328
Gene Symbol RPL15
Gene ID (NCBI) 6138
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP61313
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL15 (ribosomal protein L15) is a component of the ribosomal large subunit 60S, participating in the assembly process of ribosomal subunits and involved in the processing of rRNA. Moreover, RPL15 deletion will lead to abnormalities in the biogenesis of ribosomal subunits. The abnormal expression or mutation of RPL15 protein is also closely related to the occurrence and development of many diseases. RPL15 was highly expressed in gastric cancer cell lines, and interference with the expression of RPL15 could inhibit gastric cancer cell growth. In addition, RPL15 in skin squamous cell carcinoma tissues and pancreatic cancer cell lines decreased sharply, which had a great relationship with the survival rate. PMID:35410346

Protocols

Product Specific Protocols
WB protocol for RPL15 antibody 84900-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols