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  • Western Blot (WB) analysis of mouse splenocytes using CD19 Recombinant antibody (84848-5-RR)

    WB analysis of mouse splenocytes using 84848-5-RR

    mouse splenocytes were subjected to SDS PAGE followed by western blot with 84848-5-RR (CD19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84848-5-RR

    Affinity and Kinetic Characterization of 84848-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84848-5-RR against Mouse CD19 were performed. The affinity constant is 6.90 nM.

CD19 Recombinant antibody

CD19 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84848-5-RR
Clone No.242301B9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

CD19 antigen, 242301B9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84848-5-RR targets CD19 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein Predict reactive species
Full Name CD19 antigen
Calculated Molecular Weight60 kDa
Observed Molecular Weight80-120 kDa
GenBank Accession NumberNM_009844.2
Gene Symbol Cd19
Gene ID (NCBI) 12478
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP25918
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

Protocols

Product Specific Protocols
WB protocol for CD19 antibody 84848-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols