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  • Western Blot (WB) analysis of various lysates using CD53 Recombinant antibody (84837-1-RR)

    WB analysis using 84837-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84837-1-RR (CD53 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Daudi cells using CD53 Recombinant antibody (84837-1-RR)

    IF Staining of Daudi using 84837-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Daudi cells using CD53 antibody (84837-1-RR, Clone: 242038C5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84837-1-RR

    Affinity and Kinetic Characterization of 84837-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84837-1-RR against Human CD53 were performed. The affinity constant is below 1 pM.

CD53 Recombinant antibody

CD53 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84837-1-RR
Clone No.242038C5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Tetraspanin-25, Tetraspanin 25, MOX44, Leukocyte surface antigen CD53, Cell surface glycoprotein CD53

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inJurkat cells, Ramos cells, IM-9 cells
Positive IF/ICC detected inDaudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84837-1-RR targets CD53 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein Predict reactive species
Full Name CD53 molecule
Calculated Molecular Weight24kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberNM_000560.4
Gene Symbol CD53
Gene ID (NCBI) 963
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP19397
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD53 is also named as MOX44 and Tetraspanin-25 (Tspan-25). Tetraspanin CD53 is a member of the tetraspanin superfamily expressed exclusively within the immune compartment (PMID: 32440787). T cells depend on the phosphatase CD45 to initiate T cell receptor signaling. CD53 is shown to stabilize CD45 on the membrane and is required for optimal phosphatase activity and subsequent Lck activation. Together, CD53 as a regulator of CD45 activity required for T cell immunity (PMID: 35767951). CD53 is a marker for positively selected CD4+ CD8+ thymocytes (PMID: 11867561).

Protocols

Product Specific Protocols
WB protocol for CD53 antibody 84837-1-RRDownload protocol
IF protocol for CD53 antibody 84837-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols