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NSUN7 Recombinant antibody

NSUN7 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242358B7

Cat no : 84795-1-RR

Synonyms

FLJ14001, 242358B7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NSUN7 Recombinant antibody (84795-1-RR)

    WB analysis using 84795-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84795-1-RR (NSUN7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84795-1-RR targets NSUN7 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NSUN7 fusion protein Ag11650
Full Name NOL1/NOP2/Sun domain family, member 7
Calculated Molecular Weight 475 aa, 54 kDa
Observed Molecular Weight81~90 kDa
GenBank Accession NumberBC036568
Gene Symbol NSUN7
Gene ID (NCBI) 79730
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NSUN7 (NOP2/Sun RNA methyltransferase family member 7) is involved in mitochondrial rRNA processing in post-meiotic sperm and is predicted to act upstream of or within flagellated sperm motility and sperm mitochondrion organization. In humans, Nsun7 encodes a protein with 718-amino acid and 3697-bp belonging to the methyltransferase superfamily (PMID: 37173708). The failure to produce NSUN7 would lead to the impairment in activity and motility of sperm flagella, leading to sperm motility deficit and infertility (PMID: 25702163).

Protocols

Product Specific Protocols
WB protocol for NSUN7 antibody 84795-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols