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  • Western Blot (WB) analysis of various lysates using IARS2 Recombinant antibody (84764-5-RR)

    WB analysis using 84764-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84764-5-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IARS2 Recombinant antibody (84764-5-RR)

    IF Staining of HepG2 using 84764-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-5-RR, Clone: 242219G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IARS2 Recombinant antibody (84764-5-RR)

    IF Staining of HeLa using 84764-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IARS2 antibody (84764-5-RR, Clone: 242219G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IARS2 Recombinant antibody (84764-5-RR)

    IF Staining of HepG2 using 84764-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-5-RR, Clone: 242219G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 84764-5-RR

    Affinity and Kinetic Characterization of 84764-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84764-5-RR against Human IARS2 were performed. The affinity constant is 0.154 nM.

IARS2 Recombinant antibody

IARS2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84764-5-RR
Clone No.242219G4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Isoleucyl-tRNA synthetase, Isoleucine--tRNA ligase, mitochondrial, Isoleucine tRNA ligase, IleRS, EC:6.1.1.5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84764-5-RR targets IARS2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IARS2 fusion protein Ag10696 Predict reactive species
Full Name isoleucyl-tRNA synthetase 2, mitochondrial
Calculated Molecular Weight 1012 aa, 114 kDa
Observed Molecular Weight114 kDa
GenBank Accession NumberBC010218
Gene Symbol IARS2
Gene ID (NCBI) 55699
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NSE4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.

Protocols

Product Specific Protocols
WB protocol for IARS2 antibody 84764-5-RRDownload protocol
IF protocol for IARS2 antibody 84764-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols