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CDK5 Recombinant antibody

CDK5 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241908F10

Cat no : 84749-1-RR

Synonyms

PSSALRE, EC:2.7.11.1, Cyclin-dependent-like kinase 5, Cyclin-dependent kinase 5, Cdk 5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CDK5 Recombinant antibody (84749-1-RR)

    WB analysis using 84749-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84749-1-RR (CDK5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using CDK5 Recombinant antibody (84749-1-RR)

    IHC staining of rat brain using 84749-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84749-1-RR (CDK5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using CDK5 Recombinant antibody (84749-1-RR)

    IHC staining of rat brain using 84749-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84749-1-RR (CDK5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inNIH/3T3 cells, RAW 264.7 cells, mouse brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84749-1-RR is CDK5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDK5 fusion protein Ag28465
Full Name cyclin-dependent kinase 5
Calculated Molecular Weight 31 kDa, 33 kDa
Observed Molecular Weight 31-33 kDa
GenBank Accession NumberBC005115
Gene Symbol CDK5
Gene ID (NCBI) 1020
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.

Protocols

Product Specific Protocols
WB protocol for CDK5 antibody 84749-1-RRDownload protocol
IHC protocol for CDK5 antibody 84749-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols