Tested Applications
| Positive WB detected in | A549 cells, A375 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84723-3-RR targets Tyrosinase in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Tyrosinase fusion protein Ag34940 Predict reactive species |
| Full Name | tyrosinase (oculocutaneous albinism IA) |
| Calculated Molecular Weight | 529 aa, 60 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC027179 |
| Gene Symbol | TYR |
| Gene ID (NCBI) | 7299 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P14679 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tyrosinase is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. It's a melanocyte-specific enzyme critical for the synthesis of melanin, a process normally restricted to a post-Golgi compartment termed the melanosome. Loss-of-function mutations in tyrosinase are the cause of oculocutaneous albinism. The 60-kDa tyrosinase core polypeptide is modified in the endoplasmic reticulum (ER) by cotranslational addition of multiple N-linked glycans, producing the 70-kDa species. Complex sugar modifications in the Golgi apparatus further increases tyrosinase's molecular mass to 80 kDa, the size of the mature wild-type (WT) isoform. (PMID:10823941)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Tyrosinase antibody 84723-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
