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MGEA5 Recombinant antibody

MGEA5 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242263A6

Cat no : 84722-4-RR

Synonyms

OGA, HEXC, EC:3.2.1.169, Beta-N-acetylhexosaminidase, Beta-N-acetylglucosaminidase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MGEA5 Recombinant antibody (84722-4-RR)

    WB analysis using 84722-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84722-4-RR (MGEA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84722-4-RR

    Affinity and Kinetic Characterization of 84722-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84722-4-RR against Human MGEA5 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, JAR cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84722-4-RR targets MGEA5 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MGEA5 fusion protein Ag6405
Full Name meningioma expressed antigen 5 (hyaluronidase)
Calculated Molecular Weight 103 kDa
Observed Molecular Weight 130-140 kDa
GenBank Accession NumberBC039583
Gene Symbol MGEA5
Gene ID (NCBI) 10724
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human meningioma-expressed antigen 5 (MGEA5) has two putative domains including protein O-GlcNAcase domain and histone acetyltransferase domain, therefore it is often called bifunctional protein NCOAT. Three isoforms of MEGE5 are produced by alternative splicing. MGEA5 was found to be regulated to reduce the state of glycosylation of transcitpional activators while increasing the acetylation of histones to allow for the concerted activation of eukaryotic gene transcription, for instance, acetylation of Lys8 of histone H4 and Lys 14 of histone H3 are resulted from acetyltransferase activity. In addition, single nucleotide polymorphism in MGE5A is associated with type 2 diabetes in Mexican Americans. Two bands at 130kDa and 75 kDa could be detected using the present rabbit polyclonal antibody 14711-1-AP, which is consistent with results in a related reference (PubMed:11341771).

Protocols

Product Specific Protocols
WB protocol for MGEA5 antibody 84722-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols