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  • Western Blot (WB) analysis of various lysates using NEK4 Recombinant antibody (84654-4-RR)

    WB analysis using 84654-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84654-4-RR (NEK4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NEK4 Recombinant antibody (84654-4-RR)

    IHC staining of mouse testis using 84654-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84654-4-RR (NEK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NEK4 Recombinant antibody (84654-4-RR)

    IHC staining of mouse testis using 84654-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84654-4-RR (NEK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84654-4-RR

    Affinity and Kinetic Characterization of 84654-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84654-4-RR against Human NEK4 were performed. The affinity constant is 92.8 pM.

NEK4 Recombinant antibody

NEK4 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84654-4-RR
Clone No.242081B9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

STK2, pp12301, NRK2, NimA related protein kinase 4, 242081B9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells, HEK-293T cells, HeLa cells, NCI-H1299 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84654-4-RR targets NEK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NEK4 fusion protein Ag34929 Predict reactive species
Full Name NIMA (never in mitosis gene a)-related kinase 4
Calculated Molecular Weight 781 aa, 88 kDa
Observed Molecular Weight 95-100 kDa
GenBank Accession NumberBC063044
Gene Symbol NEK4
Gene ID (NCBI) 6787
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP51957
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neks (NIMA-related kinases) are a group of serine-threonine kinases that are related to NIMA, their ortholog from Aspergillus nidulans, which is essential for cells to enter in mitosis. NEK4 was initially identified as STK2, from serine/threonine kinase 2, in a study using a kinase specific cDNA library from human breast cancer tumors or breast cancer cells.

Protocols

Product Specific Protocols
WB protocol for NEK4 antibody 84654-4-RRDownload protocol
IHC protocol for NEK4 antibody 84654-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols