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CXCR5 Recombinant antibody

CXCR5 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241777A10

Cat no : 84639-2-RR

Synonyms

CXCR 5, CXC R5, CD185, Burkitt lymphoma receptor 1, BLR1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CXCR5 Recombinant antibody (84639-2-RR)

    WB analysis using 84639-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84639-2-RR (CXCR5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inDaudi cells, Raji cells, Ramos cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84639-2-RR targets CXCR5 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name chemokine (C-X-C motif) receptor 5
Calculated Molecular Weight 372 aa, 42 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC110352
Gene Symbol CXCR5
Gene ID (NCBI) 643
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Initially discovered in 1992 as Burkitt's lymphoma receptor 1, CXCR5 (chemokine receptor 5, also known as CD185) is a seven-transmembrane G protein-coupled receptor (GPCR) protein (PMID: 32994482). CXCR5 is essential for naïve B-cell migration to and maturation in the lymph nodes and spleen, as well as for cluster of differentiation 4 T-cell (TFH and TCM) homing and interaction with B cells (PMID: 21471443). Moreover, CXCR5 is involved in the regulation of neural stem cells (NSC) and neuronal regeneration in the mouse brain, neuronal differentiation, neurogenesis, gliogenesis, and synaptogenesis (PMID: 37460877).

Protocols

Product Specific Protocols
WB protocol for CXCR5 antibody 84639-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols