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  • Western Blot (WB) analysis of LNCaP cells using PSMA/GCPII Recombinant antibody (84615-2-RR)

    WB analysis of LNCaP using 84615-2-RR (same clone as 84615-2-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 84615-2-RR (FOLH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84615-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01454-1

    Sandwich ELISA standard curve of MP01454-1

    Sandwich ELISA standard curve of MP01454-1, Human FOLH1 Recombinant Matched Antibody Pair - PBS only. 84615-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0216. 84615-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • Sample test of MP01454-1

    Sample test of MP01454-1

    Serum of six individual healthy human donors was measured. The human FOLH1 concentration of detected samples was determined to be 38.61 ng/mL with a range of 4.93 - 152.25 ng/mL.

  • Sample test of MP01454-1

    Sample test of MP01454-1

    The mean FOLH1 concentration was determined to be 2,098.65 ng/mL in LnCap cell extract based on a 2.0 mg/mL extract load.

  • Sample test of MP01454-1

    Sample test of MP01454-1

    Saliva of six individual healthy human donors was measured. The human FOLH1 concentration of detected samples was determined to be 2.13 ng/mL with a range of 0.55 - 4.58 ng/mL.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using PSMA/GCPII Recombinant antibody (84615-2-RR)

    IF Staining of LNCaP using 84615-2-RR (same clone as 84615-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using FOLH1 antibody (84615-2-RR, Clone: 242026F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84615-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01454-1

    Cytometric bead array standard curve of MP01454-1

    Cytometric bead array standard curve of MP01454-1, FOLH1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84615-2-PBS. Detection antibody: 84615-1-PBS. Standard: Eg0216. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84615-2-RR

    Affinity and Kinetic Characterization of 84615-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84615-2-RR against Human PSMA/GCPII were performed. The affinity constant is 1.26 nM.

PSMA/GCPII Recombinant antibody, PBS Only (Capture)

PSMA/GCPII Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84615-2-PBS
Clone No.242026F4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

FOLH1, PSMA, 242026F4, Cell growth-inhibiting gene 27 protein, EC:3.4.17.21

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

84615-2-PBS targets PSMA/GCPII as part of a matched antibody pair:

MP01454-1: 84615-2-PBS capture and 84615-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PSMA/GCPII fusion protein Eg0216 Predict reactive species
Full Name folate hydrolase (prostate-specific membrane antigen) 1
Calculated Molecular Weight84KD
Observed Molecular Weight100 kDa
GenBank Accession NumberNM_004476.3
Gene Symbol PSMA
Gene ID (NCBI) 2346
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ04609
Storage Buffer PBS only, pH 7.3.
Storage ConditionsStore at -80°C.

Background Information

Prostate-specific membrane antigen (PSMA), also known as glutamate carboxypeptidase II (GCPII), is a type II transmembrane glycoprotein that has gained significant attention in the field of prostate cancer (PCa) diagnostics and therapeutics. PSMA is highly overexpressed in prostate cancer tissue, especially in aggressive, androgen-deprived, metastatic, and hormone-refractory prostate cancers. Up to 95% of prostate adenocarcinomas express PSMA receptors on their surface. Physiological expression of PSMA is observed in normal prostate epithelium, duodenum, colon, renal tubules, and sympathetic ganglia. It is also found in other tissues such as the salivary glands, lacrimal glands, and various parts of the gastrointestinal tract, though at lower levels.