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DPP4/CD26 Recombinant antibody

DPP4/CD26 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242045F5

Cat no : 84606-6-RR

Synonyms

CD26, ADCP-2, ADCP2, ADCP 2, ADABP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DPP4/CD26 Recombinant antibody (84606-6-RR)

    WB analysis using 84606-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84606-6-RR (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using DPP4/CD26 Recombinant antibody (84606-6-RR)

    WB analysis of human placenta using 84606-6-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 84606-6-RR (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84606-6-RR

    Affinity and Kinetic Characterization of 84606-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84606-6-RR against Human DPP4/CD26 were performed. The affinity constant is 1.59 nM.

Tested Applications

Positive WB detected inPC-3 cells, human placenta tissue, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84606-6-RR targets DPP4/CD26 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name dipeptidyl-peptidase 4
Calculated Molecular Weight 88 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC013329
Gene Symbol DPP4
Gene ID (NCBI) 1803
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).

Protocols

Product Specific Protocols
WB protocol for DPP4/CD26 antibody 84606-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols