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  • Western Blot (WB) analysis of various lysates using PON3 Recombinant antibody (84604-4-RR)

    WB analysis using 84604-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84604-4-RR (PON3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PON3 Recombinant antibody (84604-4-RR)

    IHC staining of mouse liver using 84604-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84604-4-RR (PON3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84604-4-RR

    Affinity and Kinetic Characterization of 84604-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84604-4-RR against Human PON3 were performed. The affinity constant is below 1 pM.

PON3 Recombinant antibody

PON3 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84604-4-RR
Clone No.242035D11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Paraoxonase 3 , Paraoxanase-3, EC:3.1.8.1, 242035D11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells, HuH-7 cells, HepG2 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84604-4-RR targets PON3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PON3 fusion protein Ag36602 Predict reactive species
Full Name paraoxonase 3
Calculated Molecular WeightNM_000940.2 40kDa，354aa
Observed Molecular Weight40 kDa
GenBank Accession NumberNM_000940.2
Gene Symbol PON3
Gene ID (NCBI) 5446
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ15166
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PON3, a member of the paraoxonase family, is expressed primarily in the liver and encodes a protein that is secreted into the bloodstream and binds to high-density lipoprotein (HDL). It also rapidly hydrolyzes lactones and inhibits the oxidation of low-density lipoprotein (LDL), thereby slowing the onset and progression of atherosclerosis. In addition, PON3 is closely associated with hepatocellular carcinoma (HCC), and is a potential therapeutic target for HCC by inducing cell cycle arrest and further inhibiting HCC cell proliferation.

Protocols

Product Specific Protocols
WB protocol for PON3 antibody 84604-4-RRDownload protocol
IHC protocol for PON3 antibody 84604-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols