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TIM-1 Recombinant antibody

TIM-1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242001D1

Cat no : 84600-5-RR

Synonyms

HAVCR1, KIM-1, KIM1, HAVcr-1, CD365

Validation Data Gallery

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  • Western Blot (WB) analysis of HuH-7 cells using TIM-1 Recombinant antibody (84600-5-RR)

    WB analysis of HuH-7 using 84600-5-RR

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 84600-5-RR (TIM-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84600-5-RR

    Affinity and Kinetic Characterization of 84600-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84600-5-RR against Human TIM-1 were performed. The affinity constant is 0.314 nM.

Tested Applications

Positive WB detected inHuH-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84600-5-RR targets TIM-1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name hepatitis A virus cellular receptor 1
Calculated Molecular Weight39kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberNM_001173393.3
Gene Symbol HAVCR1
Gene ID (NCBI) 26762
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIM-1, also known as kidney injury molecule (KIM-1), was first identified as a marker of acute kidney injury. TIM-1 mRNA and protein dramatically increase after ischemic kidney injury while there are low levels in normal kidneys. In addition, TIM-1 is expressed on CD4+ T cells after activation and its expression was sustained preferentially in Th2 but not Th1 cells. However, TIM-1 is expressed on both Th1 and Th2 cells and TIM-1 blockade prolongs survival of fully MHC-mismatched cardiac transplants. TIM-4, expressed by macrophages, is the ligand for TIM-1, and TIM-1 - TIM-4 interactions regulate Th cell responses and modulate Th1/Th2 cytokine balance. Moreover, TIM-1 can regulate macrophage activation and alter the co-stimulatory properties of macrophages. (PMID: 20091883)

Protocols

Product Specific Protocols
WB protocol for TIM-1 antibody 84600-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols