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CD46 Recombinant antibody

CD46 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241764F8

Cat no : 84599-5-RR

Synonyms

TRA2.10, TLX, MIC10, Membrane cofactor protein, MCP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD46 Recombinant antibody (84599-5-RR)

    WB analysis using 84599-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84599-5-RR (CD46 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD46 Recombinant antibody (84599-5-RR)

    IF Staining of HeLa using 84599-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD46 antibody (84599-5-RR, Clone: 241764F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84599-5-RR

    Affinity and Kinetic Characterization of 84599-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84599-5-RR against Human CD46 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inA549 cells, HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84599-5-RR targets CD46 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name CD46 molecule, complement regulatory protein
Calculated Molecular Weight42kDa
Observed Molecular Weight50-65 kDa
GenBank Accession NumberNM_172351.3
Gene Symbol CD46
Gene ID (NCBI) 4179
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).

Protocols

Product Specific Protocols
WB protocol for CD46 antibody 84599-5-RRDownload protocol
IF protocol for CD46 antibody 84599-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols