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FATP5/SLC27A5 Recombinant antibody

FATP5/SLC27A5 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241884H2

Cat no : 84557-1-RR

Synonyms

FATP5, SLC27A5, Bile acyl-CoA synthetase, Bile acid-CoA ligase, BA-CoA ligase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FATP5/SLC27A5 Recombinant antibody (84557-1-RR)

    WB analysis using 84557-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84557-1-RR (SLC27A5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using FATP5/SLC27A5 Recombinant antibody (84557-1-RR)

    IHC staining of mouse liver using 84557-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84557-1-RR (FATP5/SLC27A5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using FATP5/SLC27A5 Recombinant antibody (84557-1-RR)

    IHC staining of mouse liver using 84557-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84557-1-RR (FATP5/SLC27A5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84557-1-RR targets FATP5/SLC27A5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FATP5/SLC27A5 fusion protein Ag18276
Full Name solute carrier family 27 (fatty acid transporter), member 5
Calculated Molecular Weight 690 aa, 75 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC148807
Gene Symbol FATP5/SLC27A5
Gene ID (NCBI) 10998
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC27A5, also known as FATP5, ACSVL6, and BAL, is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty acids containing 24- and 26-carbons(PMID: 10479480). FATP5 mediates the import of long-chain fatty acids (LCFA) by facilitating their transport across cell membranes (PubMed:20448275). FATP5 is associated with endoplasmic reticulum but not with peroxisomes, and its primary role is in fatty acid elongation or complex lipid synthesis. FATP5 is exclusively expressed by the liver and localized to the basal plasma membrane of hepatocytes (PMID:16618416).

Protocols

Product Specific Protocols
WB protocol for FATP5/SLC27A5 antibody 84557-1-RRDownload protocol
IHC protocol for FATP5/SLC27A5 antibody 84557-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols