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IL1RN Recombinant antibody, PBS Only (Capture)

IL1RN Uni-rAbTM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241879G2

Cat no : 84532-5-PBS

Synonyms

IL 1ra, IL-1RA, IL1RA, IL 1RN, ICIL-1RA

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01389-3

    Sandwich ELISA standard curve of MP01389-3

    Sandwich ELISA standard curve of MP01389-3, Human IL-1RA Recombinant Matched Antibody Pair - PBS only. 84532-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1277. 84532-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP01389-3

    Sample test of MP01389-3

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean IL-1RA concentration was determined to be 4,505.9 pg/mL in unstimulated PBMC supernatant, 74,132.2 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP01389-3

    Sample test of MP01389-3

    HepG2 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human IL-1RA, and measured 534.4 pg/mL.

  • Sample test of MP01389-3

    Sample test of MP01389-3

    Serum of six individual healthy human donors was measured. The IL-1RA concentration of detected samples was determined to be 637.3 pg/mL with a range of 49.7-1,417.8 pg/mL.

Product Information

84532-5-PBS targets IL1RN as part of a matched antibody pair:

MP01389-3: 84532-5-PBS capture and 84532-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IL1RN fusion protein Ag1277
Full Name interleukin 1 receptor antagonist
Calculated Molecular Weight 20 kDa
GenBank Accession NumberBC009745
Gene Symbol IL-1RA
Gene ID (NCBI) 3557
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.