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  • Western Blot (WB) analysis of various lysates using FKBP11 Recombinant antibody (84478-5-RR)

    WB analysis using 84478-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84478-5-RR (FKBP11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using FKBP11 Recombinant antibody (84478-5-RR)

    WB analysis of mouse pancreas using 84478-5-RR

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 84478-5-RR (FKBP11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using FKBP11 Recombinant antibody (84478-5-RR)

    IF Staining of Raji using 84478-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using FKBP11 antibody (84478-5-RR, Clone: 241912G12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84478-5-RR

    Affinity and Kinetic Characterization of 84478-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84478-5-RR against Human FKBP11 were performed. The affinity constant is 0.522 nM.

FKBP11 Recombinant antibody

FKBP11 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84478-5-RR
Clone No.241912G12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

FK506 binding protein 11, EC:5.2.1.8, 241912G12, 19 kDa FKBP, 19 kDa FK506-binding protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inmouse stomach tissue, mouse pancreas tissue, rat stomach tissue, mouse liver tissue
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84478-5-RR targets FKBP11 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FKBP11 fusion protein Ag11368 Predict reactive species
Full Name FK506 binding protein 11, 19 kDa
Calculated Molecular Weight 201 aa, 22 kDa
Observed Molecular Weight24 kDa
GenBank Accession NumberBC027973
Gene Symbol FKBP11
Gene ID (NCBI) 51303
RRIDAB_3671999
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NYL4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FKBP11 (FK506 binding protein 11, also named FKBP19) was first described as predominantly expressed in secretory tissues including the pancreas and recent studies have suggested a role in beta cell survival under conditions of ER stress (PMID: 35456020). In addition, FKBP11 appears to play a role in osteoblasts and bone formation where it has been observed to associate with interferon-inducible transmembrane protein 5 (IFITM5) (PMID: 24058703).

Protocols

Product Specific Protocols
WB protocol for FKBP11 antibody 84478-5-RRDownload protocol
IF protocol for FKBP11 antibody 84478-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols