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TGFBR1 Recombinant antibody

TGFBR1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241441A11

Cat no : 84453-1-RR

Synonyms

Activin receptor-like kinase 5, Activin receptor like kinase 5, Activin A receptor type II-like protein kinase of 53kD, AAT5, 241441A11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TGFBR1 Recombinant antibody (84453-1-RR)

    WB analysis using 84453-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84453-1-RR (TGFBR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TGFBR1 Recombinant antibody (84453-1-RR)

    IHC staining of human lung cancer using 84453-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84453-1-RR (TGFBR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TGFBR1 Recombinant antibody (84453-1-RR)

    IHC staining of human lung cancer using 84453-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84453-1-RR (TGFBR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using TGFBR1 Recombinant antibody (84453-1-RR)

    IHC staining of human placenta using 84453-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84453-1-RR (TGFBR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84453-1-RR

    Affinity and Kinetic Characterization of 84453-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84453-1-RR against Human TGFBR1 were performed. The affinity constant is 67.5 pM.

Tested Applications

Positive WB detected inNCI-H1299 cells, MDA-MB-231 cells, A549 cells
Positive IHC detected inhuman lung cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84453-1-RR targets TGFBR1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TGFBR1 fusion protein Ag31620
Full Name transforming growth factor, beta receptor 1
Calculated Molecular Weight56KD
Observed Molecular Weight56 kDa
GenBank Accession NumberNM_004612
Gene Symbol TGFBR1
Gene ID (NCBI) 7046
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TGFBR1 (TGF-beta receptor type-1) encodes a serine/threonine kinase receptor for transforming growth factor-beta. TGFB1, TGFB2 and TGFB3 signals are transduced from the cell surface to the cytoplasm and regulate lots of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Mutations in both TGFBR2 and TGFBR1 were associated with early onset and aggressive thoracic aortic disease with MFS-like skeletal features, but also hypertelorism, craniosynostosis, developmental delay, cleft palate and bifid uvula, congenital heart disease and aneurysms, and dissections throughout the arterial tree with marked arterial tortuosity (PMID: 15731757, PMID: 27879313).

Protocols

Product Specific Protocols
WB protocol for TGFBR1 antibody 84453-1-RRDownload protocol
IHC protocol for TGFBR1 antibody 84453-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols