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  • Western Blot (WB) analysis of mouse splenocytes using NKp46/NCR1 Recombinant antibody (84416-5-RR)

    WB analysis of mouse splenocytes using 84416-5-RR

    mouse splenocytes were subjected to SDS PAGE followed by western blot with 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using NKp46/NCR1 Recombinant antibody (84416-5-RR)

    IHC staining of rat spleen using 84416-5-RR

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat spleen tissue using NKp46/NCR1 Recombinant antibody (84416-5-RR)

    IHC staining of rat spleen using 84416-5-RR

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat thymus tissue using NKp46/NCR1 Recombinant antibody (84416-5-RR)

    IHC staining of rat thymus using 84416-5-RR

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84416-5-RR

    Affinity and Kinetic Characterization of 84416-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84416-5-RR against Rat NKp46/NCR1 were performed. The affinity constant is 0.593 nM.

NKp46/NCR1 Recombinant antibody

NKp46/NCR1 {ptg:Brand} Recombinant Antibody for WB, IHC, ELISA
Cat No. 84416-5-RR
Clone No.241811H7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Ly94, Kilr1, CD335, Ar1, Activating receptor 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inmouse splenocytes
Positive IHC detected inrat spleen tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84416-5-RR targets NKp46/NCR1 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein Predict reactive species
Full Name natural cytotoxicity triggering receptor 1
Calculated Molecular Weight37kDa
Observed Molecular Weight50-60 kDa
GenBank Accession NumberNM_057199.1
Gene Symbol NKp46/NCR1
Gene ID (NCBI) 117547
RRIDAB_3671945
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Z0H5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets, and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).

Protocols

Product Specific Protocols
WB protocol for NKp46/NCR1 antibody 84416-5-RRDownload protocol
IHC protocol for NKp46/NCR1 antibody 84416-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols