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CCL20/MIP-3 alpha Recombinant antibody

CCL20/MIP-3 alpha Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241694B4

Cat no : 84413-3-RR

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCL20/MIP-3 alpha Recombinant antibody (84413-3-RR)

    WB analysis using 84413-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84413-3-RR (CCL20/MIP-3 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inTHP-1 cells, RAW 264.7 cells, HepG2 cells, HuH-7 cells, SMMC-7721 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84413-3-RR targets CCL20/MIP-3 alpha in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein
Full Name chemokine (C-C motif) ligand 20
Calculated Molecular Weight11kDa
Observed Molecular Weight11 kDa
GenBank Accession NumberNM_004591.3
Gene Symbol CCL20/MIP-3 alpha
Gene ID (NCBI) 6364
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCL20, also known as macrophage infiltrating factor protein 3α, is a C-C chemokine that specifically binds to the receptor CCR6. CCL20 is produced in various tissues and by a wide range of immune cells, including neutrophils, natural killer cells, T-helper type 17 (Th17) cells, B cells, and various antigen-presenting cells, such as dendritic cells (DCs), Langerhans cells, and macrophages. Increased expression of CCL20 is likely involved in the increased recruitment of dendritic cells observed in fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). CCL20 expression is increased by the proinflammatory cytokine IL-1 beta.

Protocols

Product Specific Protocols
WB protocol for CCL20/MIP-3 alpha antibody 84413-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols