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CD40 Recombinant antibody, PBS Only (Capture)

CD40 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241760A4

Cat no : 84406-1-PBS

Synonyms

p50, CD40L receptor, Bp50, B-cell surface antigen CD40, B cell surface antigen CD40

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD40 Recombinant antibody (84406-1-RR)

    WB analysis using 84406-1-RR (same clone as 84406-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84406-1-RR (CD40/TNFRSF5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84406-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD40 Recombinant antibody (84406-1-RR)

    IHC staining of human tonsillitis using 84406-1-RR (same clone as 84406-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84406-1-RR (CD40 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84406-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD40 Recombinant antibody (84406-1-RR)

    IHC staining of human tonsillitis using 84406-1-RR (same clone as 84406-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84406-1-RR (CD40 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84406-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01294-1

    Cytometric bead array standard curve of MP01294-1

    Cytometric bead array standard curve of MP01294-1, CD40 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84406-1-PBS. Detection antibody: 84406-2-PBS. Standard: Eg1797. Range: 0.625-80 ng/mL.

Product Information

84406-1-PBS targets CD40 as part of a matched antibody pair:

MP01294-1: 84406-1-PBS capture and 84406-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein
Full Name CD40 molecule, TNF receptor superfamily member 5
Calculated Molecular Weight31kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberNM_001250.6
Gene Symbol CD40
Gene ID (NCBI) 958
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.