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PURG Recombinant antibody

PURG Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241504E7

Cat no : 84399-3-RR

Synonyms

PURG B, PURG A, 241504E7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PURG Recombinant antibody (84399-3-RR)

    WB analysis using 84399-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84399-3-RR (PURG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PURG Recombinant antibody (84399-3-RR)

    IF Staining of HeLa using 84399-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PURG antibody (84399-3-RR, Clone: 241504E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84399-3-RR

    Affinity and Kinetic Characterization of 84399-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84399-3-RR against Human PURG were performed. The affinity constant is 0.328 nM.

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84399-3-RR is PURG Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PURG fusion protein Ag15820
Full Name purine-rich element binding protein G
Calculated Molecular Weight 347 aa, 40 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC106708
Gene Symbol PURG
Gene ID (NCBI) 29942
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PURG antibody 84399-3-RRDownload protocol
IF protocol for PURG antibody 84399-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols