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IL-18R1/CD218a Recombinant antibody

IL-18R1/CD218a Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241673G6

Cat no : 84390-5-RR

Synonyms

IL18R1, IL 18R1, IL 18R 1, EC:3.2.2.6, CD218a

Validation Data Gallery

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  • Western Blot (WB) analysis of NK-92 cells using IL-18R1/CD218a Recombinant antibody (84390-5-RR)

    WB analysis of NK-92 using 84390-5-RR

    NK-92 cells were subjected to SDS PAGE followed by western blot with 84390-5-RR (IL18R1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NK-92 cells using IL-18R1/CD218a Recombinant antibody (84390-5-RR)

    IF Staining of NK-92 using 84390-5-RR

    Immunofluorescent analysis of (4% PFA) fixed NK-92 cells using IL-18R1/CD218a antibody (84390-5-RR, Clone: 241673G6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84390-5-RR

    Affinity and Kinetic Characterization of 84390-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84390-5-RR against Human IL-18R1/CD218a were performed. The affinity constant is 0.948 nM.

Tested Applications

Positive WB detected inNK-92 cells
Positive IF/ICC detected inNK-92 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84390-5-RR targets IL-18R1/CD218a in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name interleukin 18 receptor 1
Calculated Molecular Weight 541 aa, 62 kDa
Observed Molecular Weight60-75 kDa
GenBank Accession NumberBC093975
Gene Symbol IL18R1
Gene ID (NCBI) 8809
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-18R1 (CD218a) is a component of the interleukin-18 receptor (IL-18R) complex, which is critical for the signaling of IL-18, a pro-inflammatory cytokine. This receptor complex, consisting of IL-18R1 and the IL-18 receptor accessory protein (IL-18RAP), belongs to the immunoglobulin-like family of interleukin-1 receptors (PMID: 35461924). IL-18R1 plays a significant role in inducing the production of interferon-gamma (IFN-γ) and other inflammatory cytokines, contributing to innate and adaptive immune responses (PMID: 2550053). It is also suggested to play a role in hypertension and chronic kidney disease, where the IL-18/IL-18R1 signaling axis may have diagnostic and therapeutic potential (PMID: 35461924).

Protocols

Product Specific Protocols
WB protocol for IL-18R1/CD218a antibody 84390-5-RRDownload protocol
IF protocol for IL-18R1/CD218a antibody 84390-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols