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PDGFR alpha/CD140a Recombinant antibody

PDGFR alpha/CD140a Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241658G6

Cat no : 84383-2-RR

Synonyms

PDGFRA, CD140a antigen, CD140A, CD140 antigen-like family member A, Alpha-type platelet-derived growth factor receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDGFRA Recombinant antibody (84383-2-RR)

    WB analysis using 84383-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84383-2-RR (PDGFRA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84383-2-RR targets PDGFR alpha/CD140a in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name platelet-derived growth factor receptor, alpha polypeptide
Calculated Molecular Weight 123 kDa
Observed Molecular Weight170-190 kDa
GenBank Accession NumberBC015186
Gene Symbol PDGFR alpha
Gene ID (NCBI) 5156
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet-derived growth factor receptor alpha (PDGFRA, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival, and chemotaxis. Mutations in the PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.

Protocols

Product Specific Protocols
WB protocol for PDGFR alpha/CD140a antibody 84383-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols