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Conjugate
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Conjugate
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Conjugate
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IKKA Recombinant antibody

IKKA Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241579D12

Cat no : 84372-5-RR

Synonyms

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using IKKA Recombinant antibody (84372-5-RR)

    IF Staining of HCT 116 using 84372-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using IKKA antibody (84372-5-RR, Clone: 241579D12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using IKKA Recombinant antibody (84372-5-RR)

    FC experiment of HepG2 using 84372-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IKKA Recombinant Antibody (84372-5-RR, Clone:241579D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHCT 116 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84372-5-RR is IKKA Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IKKA fusion protein Ag26573
Full Name conserved helix-loop-helix ubiquitous kinase
Calculated Molecular Weight 85 kDa
GenBank Accession NumberNM_001278
Gene Symbol IKK Alpha
Gene ID (NCBI) 1147
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NF‐κB is regulated by phosphorylation of one of its cytoplasmic inhibitors, IκB, mediated by IκB kinases (IKK), allowing NF‐κB nuclear translocation and transcriptional activation. IKKα‐IKKβ heterodimer complexes regulate the DNA binding proteins implicated in the canonical NF‐κB pathway, including p50‐p65, while IKKα‐homodimers regulate p52‐RELB dimers implicated in the alternative NF‐κB pathway. (PMID: 34187082)

Protocols

Product Specific Protocols
IF protocol for IKKA antibody 84372-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols