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  • Western Blot (WB) analysis of various lysates using EPN2 Recombinant antibody (84366-5-RR)

    WB analysis using 84366-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84366-5-RR (EPN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using EPN2 Recombinant antibody (84366-5-RR)

    IHC staining of mouse lung using 84366-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84366-5-RR (EPN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using EPN2 Recombinant antibody (84366-5-RR)

    IHC staining of mouse lung using 84366-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84366-5-RR (EPN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EPN2 Recombinant antibody

EPN2 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84366-5-RR
Clone No.241634F12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Epsin-2, epsin 2, EPS-15-interacting protein 2, EPS 15 interacting protein 2, EHB21

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHEK-293T cells, SH-SY5Y cells, HepG2 cells, U2OS cells, PC-3 cells, MDA-MB-231 cells, NIH/3T3 cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84366-5-RR targets EPN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EPN2 fusion protein Ag35194 Predict reactive species
Full Name epsin 2
Calculated Molecular Weight 584 aa, 62 kDa
Observed Molecular Weight62 kDa
GenBank Accession NumberBC093972
Gene Symbol EPN2
Gene ID (NCBI) 22905
RRIDAB_3671902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO95208
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPN2 (EPS 15 interacting protein 2) is a protein that interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. EPN2 is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery.

Protocols

Product Specific Protocols
WB protocol for EPN2 antibody 84366-5-RRDownload protocol
IHC protocol for EPN2 antibody 84366-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols