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RAPGEF2 Recombinant antibody

RAPGEF2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241637G5

Cat no : 84331-4-RR

Synonyms

PDZ GEF1, NRAPGEP, nRap GEP, KIAA0313, CNrasGEF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAPGEF2 Recombinant antibody (84331-4-RR)

    WB analysis using 84331-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84331-4-RR (RAPGEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RAPGEF2 Recombinant antibody (84331-4-RR)

    IHC staining of mouse kidney using 84331-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84331-4-RR (RAPGEF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using RAPGEF2 Recombinant antibody (84331-4-RR)

    IF Staining of Jurkat using 84331-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RAPGEF2 antibody (84331-4-RR, Clone: 241637G5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84331-4-RR

    Affinity and Kinetic Characterization of 84331-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84331-4-RR against Human RAPGEF2 were performed. The affinity constant is 0.19 nM.

Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, Jurkat cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84331-4-RR targets RAPGEF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAPGEF2 fusion protein Ag35288
Full Name Rap guanine nucleotide exchange factor (GEF) 2
Calculated Molecular Weight167kDa
Observed Molecular Weight200 kDa
GenBank Accession NumberNM_014247.2
Gene Symbol RAPGEF2
Gene ID (NCBI) 9693
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RAPGEF2 antibody 84331-4-RRDownload protocol
IHC protocol for RAPGEF2 antibody 84331-4-RRDownload protocol
IF protocol for RAPGEF2 antibody 84331-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols