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VIPAS39 Recombinant antibody, PBS Only (Capture)

VIPAS39 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241616A12

Cat no : 84320-2-PBS

Synonyms

C14orf133, 241616A12, Spermatogenesis-defective protein 39 homolog, SPE39, hSPE-39

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VIPAS39 Recombinant antibody (84320-2-RR)

    WB analysis using 84320-2-RR (same clone as 84320-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84320-2-RR (C14orf133 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84320-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01194-2

    Cytometric bead array standard curve of MP01194-2

    Cytometric bead array standard curve of MP01194-2, C14orf133 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84320-2-PBS. Detection antibody: 84320-3-PBS. Standard: Ag14762. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01194-3

    Cytometric bead array standard curve of MP01194-3

    Cytometric bead array standard curve of MP01194-3, C14orf133 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84320-2-PBS. Detection antibody: 84320-1-PBS. Standard: Ag14762. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84320-2-RR

    Affinity and Kinetic Characterization of 84320-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84320-2-RR against Human VIPAS39 were performed. The affinity constant is below 1 pM.

Product Information

84320-2-PBS targets VIPAS39 as part of a matched antibody pair:

MP01194-2: 84320-2-PBS capture and 84320-3-PBS detection (validated in Cytometric bead array)

MP01194-3: 84320-2-PBS capture and 84320-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen VIPAS39 fusion protein Ag14762
Full Name chromosome 14 open reading frame 133
Calculated Molecular Weight 493 aa, 57 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC015054
Gene Symbol VIPAS39
Gene ID (NCBI) 63894
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VIPAS39 (also known as C14orf133 or SPE-39) is a binding protein to Vps33B, one of the subunits in the mammalian HOPS complex. VIPAS39 may be involved in endosomal maturation or fusion (PMID: 23918659). Mutations in Vps33B and VIPAS39 cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a rare autosomal recessive multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).