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ATPBD3 Recombinant antibody

ATPBD3 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241630C4

Cat no : 84318-4-RR

Synonyms

NCS6, CTU1, 241630C4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATPBD3 Recombinant antibody (84318-4-RR)

    WB analysis using 84318-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84318-4-RR (ATPBD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATPBD3 Recombinant antibody (84318-4-RR)

    IHC staining of mouse kidney using 84318-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84318-4-RR (ATPBD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATPBD3 Recombinant antibody (84318-4-RR)

    IHC staining of mouse kidney using 84318-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84318-4-RR (ATPBD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84318-4-RR

    Affinity and Kinetic Characterization of 84318-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84318-4-RR against Human ATPBD3 were performed. The affinity constant is 0.406 nM.

Tested Applications

Positive WB detected inPC-3 cells, K-562 cells, HeLa cells, HEK-293 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84318-4-RR is ATPBD3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATPBD3 fusion protein Ag35037
Full Name ATP binding domain 3
Calculated Molecular Weight36 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberNM_145232
Gene Symbol ATPBD3
Gene ID (NCBI) 90353
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATPBD3, also known as CTU1, NCS6, is a subunit of a highly conserved protein complex involved in the thiolation of wobble U34(PMID: 18391219). ATPBD3 directly binds tRNAs and probably acts by catalyzing the adenylation of tRNAs, an intermediate required for 2-thiolation.

Protocols

Product Specific Protocols
WB protocol for ATPBD3 antibody 84318-4-RRDownload protocol
IHC protocol for ATPBD3 antibody 84318-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols