• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HNF4A Recombinant antibody

HNF4A Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241593C4

Cat no : 84316-3-RR

Synonyms

HNF4a8, HNF4a7, HNF4, HNF 4 alpha, Hepatocyte nuclear factor 4-alpha

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using HNF4A Recombinant antibody (84316-3-RR)

    WB analysis of HepG2 using 84316-3-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 84316-3-RR (HNF4A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84316-3-RR targets HNF4A in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HNF4A fusion protein Ag24647
Full Name hepatocyte nuclear factor 4, alpha
Calculated Molecular Weight 53 kDa
Observed Molecular Weight56 kDa
GenBank Accession NumberBC137539
Gene Symbol HNF4A
Gene ID (NCBI) 3172
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNF4A (Hepatocyte nuclear factor 4 α) is highly conserved between humans and mice. Using conditional knockouts, epithelial HNF4A is indispensable for protecting against acute chemical-induced colitis and spontaneous colonic inflammation in aged animals (PMID:35792863). Moreover, reduced expression of HNF4A has been reported in liver fibrosis and viral vector-mediated HNF4A restoration has previously been demonstrated to reverse features of chronic liver injury. Therefore, HNF4A has been suggested as a therapeutic target for treating liver fibrosis (PMID:34453962).

Protocols

Product Specific Protocols
WB protocol for HNF4A antibody 84316-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols