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IVNS1ABP Recombinant antibody

IVNS1ABP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241587B1

Cat no : 84268-1-RR

Synonyms

Influenza virus NS1A-binding protein, HSPC068, FLARA3, Aryl hydrocarbon receptor-associated protein 3, ARA3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IVNS1ABP Recombinant antibody (84268-1-RR)

    WB analysis using 84268-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84268-1-RR (IVNS1ABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using IVNS1ABP Recombinant antibody (84268-1-RR)

    WB analysis of mouse ovary using 84268-1-RR

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 84268-1-RR (IVNS1ABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IVNS1ABP Recombinant antibody (84268-1-RR)

    IF Staining of HeLa using 84268-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IVNS1ABP antibody (84268-1-RR, Clone: 241587B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using IVNS1ABP Recombinant antibody (84268-1-RR)

    FC experiment of HeLa using 84268-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug IVNS1ABP Recombinant antibody (84268-1-RR, Clone:241587B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84268-1-RR

    Affinity and Kinetic Characterization of 84268-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84268-1-RR against Human IVNS1ABP were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inU-251 cells, mouse ovary tissue, HEK-293 cells, HepG2 cells, HeLa cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84268-1-RR is IVNS1ABP Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IVNS1ABP fusion protein Ag6412
Full Name influenza virus NS1A binding protein
Calculated Molecular Weight 72 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC067739
Gene Symbol IVNS1ABP
Gene ID (NCBI) 10625
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for IVNS1ABP antibody 84268-1-RRDownload protocol
IF protocol for IVNS1ABP antibody 84268-1-RRDownload protocol
FC protocol for IVNS1ABP antibody 84268-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols