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CD22 Recombinant antibody

CD22 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241314B5

Cat no : 84265-5-RR

Synonyms

CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD22 Recombinant antibody (84265-5-RR)

    WB analysis using 84265-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84265-5-RR (CD22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD22 Recombinant antibody (84265-5-RR)

    IHC staining of human tonsillitis using 84265-5-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84265-5-RR (CD22 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD22 Recombinant antibody (84265-5-RR)

    IHC staining of human tonsillitis using 84265-5-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84265-5-RR (CD22 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84265-5-RR

    Affinity and Kinetic Characterization of 84265-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84265-5-RR against Human CD22 were performed. The affinity constant is 262 nM.

Tested Applications

Positive WB detected inRamos cells, Daudi cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84265-5-RR targets CD22 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name CD22 molecule
Calculated Molecular Weight 847 aa, 95 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberBC109306
Gene Symbol CD22
Gene ID (NCBI) 933
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

Protocols

Product Specific Protocols
WB protocol for CD22 antibody 84265-5-RRDownload protocol
IHC protocol for CD22 antibody 84265-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols