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METTL2 Recombinant antibody, PBS Only (Capture)

METTL2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241490A3

Cat no : 84257-1-PBS

Synonyms

METTL2B, METTL2A/B, METTL2A, Methyltransferase-like protein 2B, methyltransferase like 2B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL2 Recombinant antibody (84257-1-RR)

    WB analysis using 84257-1-RR (same clone as 84257-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84257-1-RR (METTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84257-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using METTL2 Recombinant antibody (84257-1-RR)

    IF Staining of MCF-7 using 84257-1-RR (same clone as 84257-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using METTL2 antibody (84257-1-RR, Clone: 241490A3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84257-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01163-3

    Cytometric bead array standard curve of MP01163-3

    Cytometric bead array standard curve of MP01163-3, METTL2B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84257-1-PBS. Detection antibody: 84257-3-PBS. Standard: Ag10410. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84257-1-RR

    Affinity and Kinetic Characterization of 84257-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84257-1-RR against Human METTL2 were performed. The affinity constant is 0.741 nM.

Product Information

84257-1-PBS targets METTL2 as part of a matched antibody pair:

MP01163-3: 84257-1-PBS capture and 84257-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen METTL2 fusion protein Ag10410
Full Name methyltransferase like 2B
Calculated Molecular Weight 313 aa, 36 kDa
Observed Molecular Weight36~43 kDa
GenBank Accession NumberBC107586
Gene Symbol METTL2B
Gene ID (NCBI) 55798
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.