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METTL2 Recombinant antibody

METTL2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241490A3

Cat no : 84257-1-RR

Synonyms

METTL2B, METTL2A/B, METTL2A, Methyltransferase-like protein 2B, methyltransferase like 2B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL2 Recombinant antibody (84257-1-RR)

    WB analysis using 84257-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84257-1-RR (METTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using METTL2 Recombinant antibody (84257-1-RR)

    IF Staining of MCF-7 using 84257-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using METTL2 antibody (84257-1-RR, Clone: 241490A3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84257-1-RR

    Affinity and Kinetic Characterization of 84257-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84257-1-RR against Human METTL2 were performed. The affinity constant is 0.741 nM.

Tested Applications

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells, SH-SY5Y cells, PC-3 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84257-1-RR is METTL2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen METTL2 fusion protein Ag10410
Full Name methyltransferase like 2B
Calculated Molecular Weight 313 aa, 36 kDa
Observed Molecular Weight36~43 kDa
GenBank Accession NumberBC107586
Gene Symbol METTL2B
Gene ID (NCBI) 55798
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.

Protocols

Product Specific Protocols
WB protocol for METTL2 antibody 84257-1-RRDownload protocol
IF protocol for METTL2 antibody 84257-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols