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CD97 Recombinant antibody

CD97 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241335A11

Cat no : 84255-6-RR

Synonyms

CD97 antigen, Adhesion G protein-coupled receptor E5 subunit beta, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5, ADGRE5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD97 Recombinant antibody (84255-6-RR)

    WB analysis using 84255-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84255-6-RR (CD97 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD97 Recombinant antibody (84255-6-RR)

    IHC staining of human tonsillitis using 84255-6-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84255-6-RR (CD97 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD97 Recombinant antibody (84255-6-RR)

    IF Staining of Jurkat using 84255-6-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD97 antibody (84255-6-RR, Clone: 241335A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84255-6-RR

    Affinity and Kinetic Characterization of 84255-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84255-6-RR against Human CD97 were performed. The affinity constant is 2.73 nM.

Tested Applications

Positive WB detected inU-937 cells, DU 145 cells, BGC-823 cells, SGC-7901 cells, HL-60 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84255-6-RR targets CD97 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name CD97 molecule
Calculated Molecular Weight 722 aa, 79 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC026690
Gene Symbol CD97
Gene ID (NCBI) 976
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).

Protocols

Product Specific Protocols
WB protocol for CD97 antibody 84255-6-RRDownload protocol
IHC protocol for CD97 antibody 84255-6-RRDownload protocol
IF protocol for CD97 antibody 84255-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols