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CD84 Recombinant antibody

CD84 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241342F3

Cat no : 84245-2-RR

Synonyms

Hly9-beta, Hly9 beta, hCD84, Cell surface antigen MAX.3, CD84 molecule

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD84 Recombinant antibody (84245-2-RR)

    WB analysis using 84245-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84245-2-RR (CD84 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD84 Recombinant antibody (84245-2-RR)

    IF Staining of Raji using 84245-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD84 antibody (84245-2-RR, Clone: 241342F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84245-2-RR

    Affinity and Kinetic Characterization of 84245-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84245-2-RR against Human CD84 were performed. The affinity constant is 0.409 nM.

Tested Applications

Positive WB detected inRaji cells, Daudi cells, Ramos cells, U-937 cells
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84245-2-RR targets CD84 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name CD84 molecule
Calculated Molecular Weight 345 aa, 39 kDa
GenBank Accession NumberBC020063
Gene Symbol CD84
Gene ID (NCBI) 8832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD84, also known as SLAMF5, is a member of the CD2 subfamily of the immunoglobulin receptor superfamily (PMID: 9310491). CD84 is a single-chain type-I glycoprotein composed of two extracellular Ig-like domains, a hydrophobic transmembrane region, and a cytoplasmic domain. It is broadly expressed on almost all leukocyte subsets. CD84 functions as a homophilic adhesion molecule, whose signaling can activate or inhibit leukocyte function depending on the cell type and its stage of activation or differentiation (PMID: 30522694).

Protocols

Product Specific Protocols
WB protocol for CD84 antibody 84245-2-RRDownload protocol
IF protocol for CD84 antibody 84245-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols