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CD200 Recombinant antibody

CD200 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241346E6

Cat no : 84244-2-RR

Synonyms

OX 2, MRC, MOX2, MOX1, CD200 molecule

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using CD200 Recombinant antibody (84244-2-RR)

    WB analysis of human placenta using 84244-2-RR

    human placenta tissue was subjected to SDS PAGE followed by western blot with 84244-2-RR (CD200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84244-2-RR

    Affinity and Kinetic Characterization of 84244-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84244-2-RR against Human CD200 were performed. The affinity constant is 4.34 nM.

Tested Applications

Positive WB detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84244-2-RR targets CD200 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name CD200 molecule
Calculated Molecular Weight 269 aa, 30 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC022522
Gene Symbol CD200
Gene ID (NCBI) 4345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).

Protocols

Product Specific Protocols
WB protocol for CD200 antibody 84244-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols