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TNFR1/CD120a Recombinant antibody

TNFR1/CD120a Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241336B4

Cat no : 84243-5-RR

Synonyms

CD120a, TNFR1, TNFRSF1A, 241336B4, p60

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNFR1/CD120a Recombinant antibody (84243-5-RR)

    WB analysis using 84243-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84243-5-RR (TNFR1/CD120a antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84243-5-RR

    Affinity and Kinetic Characterization of 84243-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84243-5-RR against Human TNFR1/CD120a were performed. The affinity constant is 20.5 nM.

Tested Applications

Positive WB detected inNIH/3T3 cells, HL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84243-5-RR targets TNFR1/CD120a in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name tumor necrosis factor receptor superfamily, member 1A
Calculated Molecular Weight 455 aa, 50 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC010140
Gene Symbol TNFR1
Gene ID (NCBI) 7132
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

Protocols

Product Specific Protocols
WB protocol for TNFR1/CD120a antibody 84243-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols