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CD30/TNFRSF8 Recombinant antibody

CD30/TNFRSF8 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241330G11

Cat no : 84241-2-RR

Synonyms

CD30, 241330G11, Ki 1 antigen, Ki 1, D1S166E

Validation Data Gallery

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  • Western Blot (WB) analysis of Karpas-299 cells using CD30/TNFRSF8 Recombinant antibody (84241-2-RR)

    WB analysis of Karpas-299 using 84241-2-RR

    Karpas-299 cells were subjected to SDS PAGE followed by western blot with 84241-2-RR (CD30/TNFRSF8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84241-2-RR

    Affinity and Kinetic Characterization of 84241-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84241-2-RR against Human CD30/TNFRSF8 were performed. The affinity constant is 0.391 nM.

Tested Applications

Positive WB detected inKarpas-299 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84241-2-RR targets CD30/TNFRSF8 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name tumor necrosis factor receptor superfamily, member 8
Calculated Molecular Weight 64 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberNM_001243
Gene Symbol CD30
Gene ID (NCBI) 943
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD30 is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. CD30 expression is found on activated T and B cells. CD30 is useful in diagnosis of classic Hodgkin's lymphoma, anaplastic large cell lymphoma and embryonal carcinoma.

Protocols

Product Specific Protocols
WB protocol for CD30/TNFRSF8 antibody 84241-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols