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CD79a Recombinant antibody

CD79a Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241299F1

Cat no : 84236-4-RR

Synonyms

MB 1, Ig-alpha, IGA, Ig alpha, B-cell antigen receptor complex-associated protein alpha chain

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD79a Recombinant antibody (84236-4-RR)

    WB analysis using 84236-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84236-4-RR (CD79a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD79a Recombinant antibody (84236-4-RR)

    IHC staining of human tonsillitis using 84236-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84236-4-RR (CD79a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD79a Recombinant antibody (84236-4-RR)

    IHC staining of human tonsillitis using 84236-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84236-4-RR (CD79a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84236-4-RR

    Affinity and Kinetic Characterization of 84236-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84236-4-RR against Human CD79a were performed. The affinity constant is 20.8 nM.

Tested Applications

Positive WB detected inDaudi cells, Ramos cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84236-4-RR targets CD79a in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name CD79a molecule, immunoglobulin-associated alpha
Calculated Molecular Weight 226 aa, 25 kDa
Observed Molecular Weight40-50 kDa
GenBank Accession NumberBC113733
Gene Symbol CD79A
Gene ID (NCBI) 973
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cells and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

Protocols

Product Specific Protocols
WB protocol for CD79a antibody 84236-4-RRDownload protocol
IHC protocol for CD79a antibody 84236-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols