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SNX18 Recombinant antibody

SNX18 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241537A2

Cat no : 84215-1-RR

Synonyms

Sorting nexin-18, SH3PXD3B, SH3 and PX domain-containing protein 3B, 241537A2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SNX18 Recombinant antibody (84215-1-RR)

    IF Staining of MCF-7 using 84215-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SNX18 antibody (84215-1-RR, Clone: 241537A2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84215-1-RR is SNX18 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SNX18 fusion protein Ag16645
Full Name sorting nexin 18
Calculated Molecular Weight 628 aa, 69 kDa
GenBank Accession NumberBC060791
Gene Symbol SNX18
Gene ID (NCBI) 112574
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).

Protocols

Product Specific Protocols
IF protocol for SNX18 antibody 84215-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols