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CD90/Thy1 Recombinant antibody

CD90/Thy1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241388E2

Cat no : 84206-3-RR

Synonyms

Thy-1 membrane glycoprotein, Thy-1 antigen, Thy-1, Thy1, CD90

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD90/Thy1 Recombinant antibody (84206-3-RR)

    WB analysis using 84206-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84206-3-RR (CD90/Thy1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using CD90/Thy1 Recombinant antibody (84206-3-RR)

    IF Staining of PC-12 using 84206-3-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CD90/Thy1 antibody (84206-3-RR, Clone: 241388E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84206-3-RR

    Affinity and Kinetic Characterization of 84206-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84206-3-RR against Rat CD90/Thy1 were performed. The affinity constant is 27.1 pM.

Tested Applications

Positive WB detected inPC-12 cells, rat brain tissue
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84206-3-RR is CD90/Thy1 Fusion Protein expressed in E. coli.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name Thy-1 cell surface antigen
Calculated Molecular Weight18 kDa
Observed Molecular Weight25 kDa
GenBank Accession NumberNM_012673.2
Gene Symbol Thy1
Gene ID (NCBI) 24832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.

Protocols

Product Specific Protocols
WB protocol for CD90/Thy1 antibody 84206-3-RRDownload protocol
IF protocol for CD90/Thy1 antibody 84206-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols