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CDK6 Recombinant antibody

CDK6 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241022F11

Cat no : 84200-1-RR

Synonyms

EC:2.7.11.22, Cyclin-dependent kinase 6, Cyclin dependent kinase 6, Cell division protein kinase 6, CDKN6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK6 Recombinant antibody (84200-1-RR)

    WB analysis using 84200-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84200-1-RR (CDK6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84200-1-RR

    Affinity and Kinetic Characterization of 84200-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84200-1-RR against Human CDK6 were performed. The affinity constant is 15.3 nM.

Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84200-1-RR is CDK6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDK6 fusion protein Ag5600
Full Name cyclin-dependent kinase 6
Calculated Molecular Weight 326 aa, 36 kDa
Observed Molecular Weight 36-40 kDa
GenBank Accession NumberBC027989
Gene Symbol CDK6
Gene ID (NCBI) 1021
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).

Protocols

Product Specific Protocols
WB protocol for CDK6 antibody 84200-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols