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Geminin Recombinant antibody

Geminin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240835B5

Cat no : 84199-2-RR

Synonyms

GMNN, MGORS6, Gem, 240835B5

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Geminin Recombinant antibody (84199-2-RR)

    WB analysis of Jurkat using 84199-2-RR

    Jurkat cells were subjected to SDS PAGE followed by western blot with 84199-2-RR (GMNN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Geminin Recombinant antibody (84199-2-RR)

    WB analysis of HEK-293 using 84199-2-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 84199-2-RR (GMNN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Geminin Recombinant antibody (84199-2-RR)

    IF Staining of HepG2 using 84199-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Geminin antibody (84199-2-RR, Clone: 240835B5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Geminin Recombinant antibody (84199-2-RR)

    IF Staining of HeLa using 84199-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Geminin antibody (84199-2-RR, Clone: 240835B5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84199-2-RR

    Affinity and Kinetic Characterization of 84199-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84199-2-RR against Human Geminin were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84199-2-RR is Geminin Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Geminin fusion protein Ag1076
Full Name geminin, DNA replication inhibitor
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 28-29 kDa
GenBank Accession NumberBC005185
Gene Symbol GMNN
Gene ID (NCBI) 51053
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in inhibiting DNA replication by preventing the incorporation of MCM complex into the pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to the apparent mass of 42.5 kDa and 66 kDa are present. The dimer of geminin(50 kDa) can also be detected. (PMID: 15313623)

Protocols

Product Specific Protocols
WB protocol for Geminin antibody 84199-2-RRDownload protocol
IF protocol for Geminin antibody 84199-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols